About this project
The objective of the PhD project is to develop colon-derived organoids that recapitulate the cystinosis phenotype of variable CTNS mutants using CRISPR/Cas9 technology on healthy colon organoid lines. These organoids will be thoroughly characterized for their cystinosis phenotype, compared with cystinosis tubuloids and cultured as microtissue in in-house designed microfluidic devices suitable for drug screening in a medium throughput fashion using 3D fluoresecent imaging. Drugs will be tested for their ability to restore the cystinosis phenotype in organoids and tubuloids.
Lead Supervisor Roos Masereeuw
Host Institution
Secondments
Mobility is an essential part of MSCA PhD projects. Secondments will undertaken at the following organisations:
- Fciencias.id – Associacao para a Investigacao e Desenvolvimento de Ciencias – Lisbon, Portugal
(12 months) - AstraZeneca AB, Sweden
(3 months)
Degree awarding institutions
Ann-Engelke Timm
DC4