About this project
The objective of this PhD project is to develop gene based therapeutic approaches for cystinosis in advanced organoid based models. The PhD candidate will use gene editing (CRISPR-Cas), to build an iPSC derived kidney organoid cystinosis model. Additionally, 3D models like microfluidic plates, hollow fiber membranes, and/or coaxially printed proximal tubules will be used to further model tubulopathy.
The PhD candidate will try to restore gene function in both gut and kidney organoids and developed 3D models. Different assays will be used to evaluate disease genotype for cystinosis and to assess efficacy and safety of the different therapies. We aim to improve gene therapy delivery, which is currently a bottleneck for genetic kidney diseases.
Lead Supervisor: Anne Metje van Genderen
Host Institution
Secondments
Mobility is an essential part of MSCA PhD projects. Secondments will undertaken at the following organisations:
- Katholieke Universiteit Leuven – Leuven, Belgium (12 months)
- Mercurna BV – Oss, Netherlands (1 month)
Degree awarding institutions
Supervisory Team
- Dr. Anne Metje van Genderen (Uutrecht)
- Prof. Rik Gijsbers (KU Leuven)
Lena Heer
DC10