About this project
The aim of this PhD project is to use state-of-the-art CRISPR-Cas9 genetic perturbations of primary airway 2D- and 3D-cell models for assay development and structure-function analyses in the context of the genetic disease Cystic Fibrosis (CF).
The PhD candidate will use conventional CRISPR-Cas9 and base-editing technologies for (large-scale) targeted manipulation of CFTR, followed by molecular and functional typing of cells using label-free, high-throughput robotic screening platforms and high-resolution imaging technologies.
Lead Supervisor: Jeff Beekman
Host Institution
Secondments
Mobility is an essential part of MSCA PhD projects. Secondments will undertaken at the following organisations:
- Fciencias.id – Associacao para a Investigacao e Desenvolvimento de Ciencias – Lisbon, Portugal
(12 months) - AstraZeneca AB, Sweden
(3 months)
Degree awarding institutions
Aditi Shekhar
DC6